bm2 agar plates  (Difco)

Journal: BMC Infectious Diseases

Article Title: Choline metabolism modulates cyclic-di-GMP signaling and virulence of Pseudomonas aeruginosa in a macrophage infection model

doi: 10.1186/s12879-024-10375-3

Figure Lengend Snippet: Cho and GB interfere c-di-GMP regulated phenotypes in P. aeruginosa. Biofilm formation of PAO1 and betAB mutant after cultured overnight with 0.1mM Cho or GB in ABTG medium ( A ). Poverdine production of PAO1 and betAB mutant after cultured overnight with 0.1mM Cho or GB in ABTG medium ( B ). Swarming motility of PAO1 and betAB mutant on BM2 plates supplemented with 0.1 mM Cho or GB, significance evaluation was listed on the right ( C ). Data was presented as mean ± SD of four biological replicates. Statistical analysis was conducted by One Way-ANOVA, ** Indicates p < 0.01, *** Indicates p < 0.001

Article Snippet: Swimming motility was assayed as previously described on BM2 agar plates (0.4% [wt/vol] glucose, 62 mM potassium phosphate buffer [pH = 7], 0.3% [wt/vol] Difco agar, 2 mM MgSO 4 , 10 M FeSO 4 , 0.1% [wt/vol] Casamino Acids) with 0.1 mM GB, 0.1 mM Cho or not [ ].

Techniques: Mutagenesis, Cell Culture

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Inhibition of Bacterial Biofilm Formation and Swarming Motility by a Small Synthetic Cationic Peptide

doi: 10.1128/AAC.00064-12

Figure Lengend Snippet: Bacterial swarming in the presence of 1037. Swarming was examined on BM2-swarm plates containing 0.5% (wt/vol) agar (Difco) after incubation for 20 h at 37°C. Swarming colonies were quantified as described in Materials and Methods. All experiments were done at least 3 times, and statistical significance was determined using one-way ANOVA (***, P < 0.001).

Article Snippet: Swarming experiments were performed on BM2 swarming agar plates (62 mM potassium phosphate buffer [pH 7], 2 mM MgSO 4 , 10 μM FeSO 4 , 0.4% [wt/vol] glucose, 0.1% [wt/vol] Casamino Acids [0.5% Casamino Acids for PAO1], 0.5% [wt/vol] Difco agar) supplemented with different concentrations of the peptide.

Techniques: Incubation